| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.079159 |
| Chromosome: | chromosome 5 |
| Location: | 3368237 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g800577 | (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCGTATTATCAAGCAAGACAAATCGCTAGCGCACGTGTTTTTGGAAGA |
| Internal bar code: | AAGGGTTGCAACAGGTGGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1610 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTATCAGCGCCATTAGGT |
| Suggested primer 2: | GGTGAAGGTGAACGGCAAAC |