| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.079163 |
| Chromosome: | chromosome 2 |
| Location: | 7162403 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390356 | (1 of 1) PTHR22849//PTHR22849:SF0 - WDSAM1 PROTEIN // WD REPEAT, SAM AND U-BOX DOMAIN-CONTAINING PROTEIN 1 | gene_edge/mRNA_edge/5'UTR | |
| Cre09.g390430 | (1 of 1) K13108 - smad nuclear-interacting protein 1 (SNIP1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAACCGGAGCAATCCATGAAGTCAAAGTTAGGCCAGACACTGACCCTG |
| Internal bar code: | GACTACTTGTGACCTACAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2612 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCAGTAGCCTGAGATCTG |
| Suggested primer 2: | ACTCCAGTAACTGCCACACG |