Insertion junction: CLIP2.079169_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.079169
Chromosome:plastome
Location:23216
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802273 2717054,ChreCp011,rpl23 (1 of 1) PTHR11620//PTHR11620:SF20 - 60S RIBOSOMAL PROTEIN L23A // 50S RIBOSOMAL PROTEIN L23, CHLOROPLASTIC; 50S ribosomal protein L23 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATAGTAATCTTTTGCTTATTTTAGAAGCAAGTATCATATAAATAAAATTG
Internal bar code:AATTAATTTTCGATCGCAGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:74
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCAATGCGAGCATTACGGTT
Suggested primer 2:ACGCATATTCCACCACGTCA