| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.079173 |
| Chromosome: | chromosome 2 |
| Location: | 795392 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g800172 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGCATCCGAGCGCCCTCAAAACATACAACAGCAGCCGTAGGAGCAATG |
| Internal bar code: | CTAGAAATGCTGCTGCTTCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1086 |
| LEAP-Seq percent confirming: | 16.6667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGGCATCGCTAGCTCTGC |
| Suggested primer 2: | CGTTGAATGTTTGGCAGGCA |