| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.079177 |
| Chromosome: | chromosome 16 |
| Location: | 2368878 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659400 | (1 of 1) PTHR23257:SF347 - SERINE/THREONINE-PROTEIN KINASE KINX-RELATED | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCAGCCACCAGCGCCGGCCCGTCAGTTCCACACACGGCGTTGCCCGAT |
| Internal bar code: | AACCACGCTAAATCACTCGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4339 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCTGGTGAGCGTGTTGT |
| Suggested primer 2: | GCCATTGCCATGCTTACGAG |