Insertion junction: CLIP2.079205_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.079205
Chromosome:chromosome 3
Location:5928414
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g188800 Nicotinate phosphoribosyltransferase; (1 of 1) PTHR11098//PTHR11098:SF2 - NICOTINATE PHOSPHORIBOSYLTRANSFERASE // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCACCCGCGACCCGGCACTCACAGATGTGCCTGCCGCTGCTTCGCAACT
Internal bar code:TGGTGCGCGATTTGCAACTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1885
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CCATCACCGGCTTTTCTCCT
Suggested primer 2:CTGTGATGACTAGCGAGCGT