| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.079218 |
| Chromosome: | chromosome 10 |
| Location: | 3344178 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTATCCTCAGCATCAACCGTTTACAGCGTCGTGTGTCTGAGCCTGCCC |
| Internal bar code: | AACTTGGCGCAAACTATTGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 54 |
| LEAP-Seq percent confirming: | 16.6667 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACAAATAAGCGACGCCG |
| Suggested primer 2: | CGCGTTTTGAACTGCACTCA |