| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.079219 |
| Chromosome: | chromosome 1 |
| Location: | 266829 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g800000 | (1 of 1) PTHR13271//PTHR13271:SF9 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGATCGCTGTTCAACAAGTACCCCTTCGACCTGCCCACCGAGGCCTTCA |
| Internal bar code: | ACTACATTTATGGGGCGGCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 142 |
| LEAP-Seq percent confirming: | 8.57143 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTATGGTAGCAGCTCACG |
| Suggested primer 2: | AATTGTCCTCTTGCTCCCCG |