| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.079220 |
| Chromosome: | chromosome 2 |
| Location: | 329540 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g075150 | RPB2 | (1 of 1) K03010 - DNA-directed RNA polymerase II subunit RPB2 (RPB2, POLR2B); DNA-directed RNA polymerase II, 135 kDa polypeptide | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTAGGTGTACTACATAGCAACTACCTACAGAGGACATAGATGTGCTG |
| Internal bar code: | GTCACTTGATTGCGCGTCCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1206 |
| LEAP-Seq percent confirming: | 61.0169 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCGTCGAAAAAGGCGGTA |
| Suggested primer 2: | TCTTGGTTCTCATGGCACCC |