| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.079228 |
| Chromosome: | chromosome 12 |
| Location: | 5908410 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533650 | (1 of 1) PTHR13439//PTHR13439:SF0 - CT120 PROTEIN // BCDNA.GH12326 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTATTAAGTATACCGTATTCAAGGCAGCATATGCTCTGCATGGCCCCT |
| Internal bar code: | GGCCGTTAAGGCATTGAGACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2961 |
| LEAP-Seq percent confirming: | 79.4118 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTGGCGGCATAAGAATG |
| Suggested primer 2: | GAATGGGCAGTTGTTTGCGT |