| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.079257 |
| Chromosome: | chromosome 11 |
| Location: | 1346880 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467710 | VSP1 | Hydroxyproline-rich cell wall glycoprotein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGATTGATTCAAACCCGTAACCGTGTGACGTTGACTAAGTTCGTGTGCG |
| Internal bar code: | TCAACTCTACAGCGGGGTCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1069 |
| LEAP-Seq percent confirming: | 47.0588 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTTGAAGCAGTTGCCCC |
| Suggested primer 2: | TGTCAACCGCCAGCAGTTAT |