Insertion junction: CLIP2.079271_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.079271
Chromosome:chromosome 3
Location:4298227
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g800329 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCGAATCTGCGCATAGCGAGGGGGAGCCCTGCTCCTCGTCATTGCGCC
Internal bar code:ATCCTTAAAGTTACATGATTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1321
LEAP-Seq percent confirming:78.7879
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:TACCCGTTGACTTGCTTCCC
Suggested primer 2:ACAGGGGTGCTTCATCTGTG