Insertion junction: CLIP2.079273_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.079273
Chromosome:chromosome 12
Location:7867213
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g553600 (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAATGCCGACATGCCCCTCCCCAAACTGCCCCAAACTATGCACGTGTGCT
Internal bar code:GAGGCACTTGCGGTGTGCGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:2232
LEAP-Seq percent confirming:16.6667
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CGACTTTGCTGCAAACCCTC
Suggested primer 2:GATGAGGCGTCGAGGACATT