Insertion junction: CLIP2.079274_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.079274
Chromosome:chromosome 16
Location:4405584
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g668350 (1 of 1) PF07889 - Protein of unknown function (DUF1664) (DUF1664) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCGAATGCACGCTGCGGCGCCGGGCCCGAAGCGGTGGTCTTCCCCGGC
Internal bar code:TTTCTGTATCACGCTAGTCAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:4285
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:117
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:117

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGTACAGCTGTGCTTGGA
Suggested primer 2:CTCGAAGGGTTCAAGCAGGT