| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.079276 |
| Chromosome: | chromosome 5 |
| Location: | 2932159 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755247 | (1 of 1) IPR003010//IPR020683 - Carbon-nitrogen hydrolase // Ankyrin repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATCGGGTTATTGGCAATCTGAATTAACATGTCGGCGCTGTCAGGCGAC |
| Internal bar code: | GCGAGCATAGGTGCTCTGATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 203 |
| LEAP-Seq percent confirming: | 40.5405 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTATCCTCGCTCAGACC |
| Suggested primer 2: | TGGGCGGATGTTATTTGGCT |