Insertion junction: CLIP2.079287_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.079287
Chromosome:chromosome 17
Location:6408767
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre17.g743947 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGTGCGGGCGAGCAAGCGGTTCTCTGATGTAGTTTAAACTAAACCTAA
Internal bar code:CGCACATCTGAGGGCGCATGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:284
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGTAGGTGCCGTTGATGGTG
Suggested primer 2:TGTATCCTAGCATGGCAGCG