| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.079289 |
| Chromosome: | chromosome 9 |
| Location: | 383854 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749847 | DLD1 | Dihydrolipoyl dehydrogenase; (1 of 1) PTHR22912:SF151 - DIHYDROLIPOYL DEHYDROGENASE, MITOCHONDRIAL | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTCCACACATTCCCCCCACGGGACCTGCACACGTCACAGCACCCGGGA |
| Internal bar code: | CGGTATAACTCGCTGCTCACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 577 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTGCAGGTCAAGATCGT |
| Suggested primer 2: | ATCGTTGAGTCTGCACCCAG |