Insertion junction: CLIP2.079294_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.079294
Chromosome:chromosome 1
Location:1991264
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g010900 GAPA1,GAP3 (1 of 1) 1.2.1.13 - Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating) / Triosephosphate dehydrogenase (NADP+); Glyceraldehyde-3-Phosphate Dehydrogenase A 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCGACAAACCACGACACCAACCACGAACCGAATCCCTCACCCGCGTGCG
Internal bar code:CGCTTTAAGAATTTGCATCGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3991
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCGCTTACCGAATTCTTG
Suggested primer 2:ATTTGTCGTGGGTTTGCGTG