| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.079296 |
| Chromosome: | chromosome 1 |
| Location: | 7023769 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g800128 | (1 of 4) PTHR11229:SF5 - 50S RIBOSOMAL PROTEIN L3-1, CHLOROPLASTIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACACGCCCCTCCGTAACCCCCTCCACGAACCGCTAGCAGTGGCAGCGC |
| Internal bar code: | TATTGCACATATCGTGGTGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1888 |
| LEAP-Seq percent confirming: | 37.8378 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGAAAGTGGGGTAGTGGC |
| Suggested primer 2: | CTAGGCCGACTGCTAACGAG |