Insertion junction: CLIP2.079303_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.079303
Chromosome:chromosome 6
Location:905543
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g255950 (1 of 1) K14553 - U3 small nucleolar RNA-associated protein 18 (UTP18) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGCCTTCGCTACGCGGTCGCTACATTCACAGCTACTGCGGACTTACTC
Internal bar code:CTTTGCTGTAAAGTTGACATTC

Confirmation - LEAP-Seq

LEAP-Seq distance:278
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ACTATGTGCACAGCCTGGAC
Suggested primer 2:GCGTAGGCACCTAACCTTGT