Insertion junction: CLIP2.079309_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.079309
Chromosome:plastome
Location:9206
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802265 ChreCp003,2716966,chlB (1 of 1) K04039 - light-independent protochlorophyllide reductase subunit B (chlB); light-independent protochlorophyllide reductase subunit B CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCATTTGTGTCCCAAAAATGGCTGCAGGTTCTAATTGTGCAATCATATC
Internal bar code:GTGTGGGGCAATCTTCAAGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:309
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CCGAAGGCGACGTCATTAGA
Suggested primer 2:TTCGAACCTGCGACCCTATG