Insertion junction: CLIP2.079315_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.079315
Chromosome:chromosome 15
Location:5364810
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre17.g734250 (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGTCCCCAGCATGCCGCACAGCACCACGCCGCTGCCGCCGTCGCCCAGC
Internal bar code:TCCCTCCTTGAACGTACATAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:72
LEAP-Seq percent confirming:10.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:AGTCGCTCATGTCCACACTG
Suggested primer 2:GACGTGTGACCAAAAGCACC