| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.079321 |
| Chromosome: | chromosome 12 |
| Location: | 2366949 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507900 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTAGCTGAACCAAGGATGAAGGTTAACCAAACCGCATTTTGCGTTGCAC |
| Internal bar code: | AGGCGGAGCAAAAGCGGTCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4220 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAATAAAGCAGCCGCCAGC |
| Suggested primer 2: | ATGCTGACTTGCTGCTGTCT |