Insertion junction: CLIP2.079344_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.079344
Chromosome:chromosome 12
Location:2260704
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g508550 PANB,PAN2,PANB1 (1 of 1) 2.1.2.11 - 3-methyl-2-oxobutanoate hydroxymethyltransferase / Ketopantoate hydroxymethyltransferase; Ketopantoate hydroxymethyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCTCGCATGATCTTTGCGGCTGAAGAAATTAGCGTGTGAGTGATGACA
Internal bar code:TATAGCTGAATTGTGTTCAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:1531
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GGAAGCTGTTTGGTTGCCAG
Suggested primer 2:ACCCCCACCATGATACCGTA