| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.079348 |
| Chromosome: | chromosome 16 |
| Location: | 1542521 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g653350 | (1 of 1) 3.5.1.108 - UDP-3-O-acyl-N-acetylglucosamine deacetylase / UDP-3-O-acyl-GlcNAc deacetylase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCATGGTGTCTTTAACAAGCCTGCTAGTGGCTTTTTTCTCCTGGCTGC |
| Internal bar code: | TAGTGCCTCAACTACGTGCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1299 |
| LEAP-Seq percent confirming: | 9.09091 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCACAGGGAAGGAGAAT |
| Suggested primer 2: | CGCGCATTGTGCATTCTACA |