| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.079350 |
| Chromosome: | chromosome 13 |
| Location: | 4301606 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g592450 | COPE1 | (1 of 1) K17268 - coatomer protein complex, subunit epsilon (COPE); Epsilon subunit of COP-I complex | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGCTGGCAAACTACCCAGGAGAGCCACTGCGCTGCAGAGAAGCAGTGG |
| Internal bar code: | TCGAGCAGATATTTAAGGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 827 |
| LEAP-Seq percent confirming: | 68.4211 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGCAGGTCAAGGTAGGTC |
| Suggested primer 2: | TACAGTGACGCCTGTGTGAC |