| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.079366 |
| Chromosome: | chromosome 12 |
| Location: | 1662309 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486550 | (1 of 31) IPR003590 - Leucine-rich repeat, ribonuclease inhibitor subtype | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGACTTGAACCACATGACCATGGCGTTCCCCACGGCCTTTCCAACACGG |
| Internal bar code: | AGCCTATGCATAATGTGGTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 682 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAACTGACGCAAGCATCA |
| Suggested primer 2: | CCTTGTCTGTGGCGTCTCTT |