| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.079370 |
| Chromosome: | chromosome 17 |
| Location: | 2992698 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g719771 | (1 of 4) PF12576 - Protein of unknown function (DUF3754) (DUF3754) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCAACGTCGCACGGCATCCCACTTCATGTCATGTATGCACCCAGGTGT |
| Internal bar code: | TACTTGGCAGAGAGGTCCCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 101 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAAAAGCAGCAGAAGCA |
| Suggested primer 2: | CGCTTGCTGTAAATGGCCAG |