Insertion junction: CLIP2.079371_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.079371
Chromosome:chromosome 7
Location:5220607
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g349100 HEL39 ATP-dependent RNA helicase; (1 of 1) K12813 - pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 (DHX16) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAGTTGAGGTGGATTGCTTTGATGCAGTTGGAACGGAGCACGGGCCGG
Internal bar code:TGCTGGTAGATATGACTTTGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2125
LEAP-Seq percent confirming:93.1034
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:TTGAAAGCGCACGTCAACAG
Suggested primer 2:TACCGCACCATCAAGAACCC