| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.079379 |
| Chromosome: | chromosome 16 |
| Location: | 7021397 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673729 | CPN11 | Chaperonin 11, chloroplastic; (1 of 1) PTHR10772:SF13 - CHLOROPLAST CHAPERONIN 10 | 3'UTR |
| Cre16.g673841 | (1 of 1) PTHR13481:SF0 - UPF0454 PROTEIN C12ORF49 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCTAGCAACCTAGAAACCAGTAGTAGGAGAAGTTAGGTGAGTATAGA |
| Internal bar code: | CAGAGTGTTGTCACCTCAGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4797 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 78 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCCGTCGAGTAGCTAAG |
| Suggested primer 2: | GTTGCTGGCGGATATTGCTG |