| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.079380 |
| Chromosome: | chromosome 10 |
| Location: | 378528 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g420500 | (1 of 6) PTHR10117 - TRANSIENT RECEPTOR POTENTIAL CHANNEL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGCACATCAGCAGCATCAGACACAGGGTTGATGGCGTGTGAGTACGTG |
| Internal bar code: | AAATTGCCTGAACATAGGATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 216 |
| LEAP-Seq percent confirming: | 30.8824 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACATGCACACACACATGC |
| Suggested primer 2: | TCCCTCCTTCCTCATCCCTG |