| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.079381 |
| Chromosome: | chromosome 10 |
| Location: | 1925251 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431650 | (1 of 2) 2.7.1.86 - NADH kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCCACACCAACCCGCCCTCTCACACCCTCAAGCAGCACCGCTGAGCCC |
| Internal bar code: | TAATTTTTCTATATATACCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 605 |
| LEAP-Seq percent confirming: | 44.4444 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGCGGTCATGTTTGTTT |
| Suggested primer 2: | AGAACGCCTGCACATTACCA |