Insertion junction: CLIP2.079383_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.079383
Chromosome:chromosome 5
Location:2552082
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g239450 (1 of 3) K14165 - atypical dual specificity phosphatase [EC:3.1.3.16 3.1.3.48] (K14165) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCCCGCAAGCGAGACACAGTTTGCCAAGCTGGATGGATGGCTGCAATC
Internal bar code:CATGTAAGCTCGTGTTTCGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:639
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGAGGAAGCCTGTTGTGC
Suggested primer 2:CGCGGCTTCAAAGGTTTTCA