| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.079398 |
| Chromosome: | chromosome 12 |
| Location: | 3821194 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g514800 | (1 of 2) K06287 - septum formation protein (maf) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAAACTAGTACATAAGGACTGGTCACTGGTGGAGTTTACAGTACACGT |
| Internal bar code: | GCTCGATCCTTTTTAAGAGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1667 |
| LEAP-Seq percent confirming: | 27.2727 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTTCTTTCTGGGCTCTG |
| Suggested primer 2: | CAACCTGTGTGTTGCATGGG |