Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.079417 |
Chromosome: | chromosome 15 |
Location: | 5561727 |
Confidence (%): | 63 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre46.g761047 | (1 of 3) IPR000104//IPR027417 - Antifreeze protein, type I // P-loop containing nucleoside triphosphate hydrolase | outside_mRNA |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGCCGCCAACCCCGGGCCAGCCACCCCCACCACAGCTGCCCGCTCCC |
Internal bar code: | ATGGTATACAGTGTATATCGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 60 |
LEAP-Seq percent confirming: | 28.5714 |
LEAP-Seq n confirming: | 2 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAAAGTGTGTTCCCAAGGCG |
Suggested primer 2: | CCAACCCCACCATTCTCTCC |