| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.079440 |
| Chromosome: | chromosome 10 |
| Location: | 363195 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g420450 | (1 of 2) PTHR18841:SF0 - PROTEIN C08G5.3, ISOFORM A-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAAGACGTCCTGTTGGGTTGCAGGGCAAGACGCGGTCGGGTCAGGCAG |
| Internal bar code: | TTTTAAACAACTACTCGGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 160 |
| LEAP-Seq percent confirming: | 46.1538 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTCAACCTGTCATCACCG |
| Suggested primer 2: | TCCACGATCTTCCACTTGCC |