| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.038403 |
| Chromosome: | chromosome_4 |
| Location: | 1999366 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g218800 | THB3 | Truncated hemoglobin | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCTGATGGCGTTGGGGCTAAGGACTGAGGA |
| Internal bar code: | ATGGTTGTCCCTTCACGTCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 846 |
| LEAP-Seq percent confirming: | 99.5526 |
| LEAP-Seq n confirming: | 890 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGTCAACCGACTGCTCT |
| Suggested primer 2: | GCTACGCTTCAGAATTTGCC |