| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.038412 |
| Chromosome: | chromosome_1 |
| Location: | 3954555 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g025750 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGGCGCCCGTGTGCAGCCTTGCAAAGCTA |
| Internal bar code: | TAATCCTTCAGGCATCGCGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 758 |
| LEAP-Seq percent confirming: | 80.5535 |
| LEAP-Seq n confirming: | 2183 |
| LEAP-Seq n nonconfirming: | 527 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTAGCCTTGCCCTCCTCA |
| Suggested primer 2: | AACCTGACTACGGATGTGCC |