| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.038422 |
| Chromosome: | chromosome 16 |
| Location: | 3946253 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690509 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCTGATTCAGGGCCGTACGGAGATACA |
| Internal bar code: | ATGGGGGAGTAGATCTTGTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 363 |
| LEAP-Seq percent confirming: | 99.1037 |
| LEAP-Seq n confirming: | 5860 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATACGGGTTTCCCTAAA |
| Suggested primer 2: | TGCTCATCAGCAATTTAGCG |