Insertion junction: LMJ.RY0402.038423_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.038423
Chromosome:chromosome_9
Location:2142911
Confidence (%):95
Locus systemic id Locus common name Defline Orientation Feature
Cre09.g393450 FAP233 Flagellar Associated Protein sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):CGTTCCAGTCGGCGGACTGCGAGTTGCAGA
Internal bar code:CGGACGTTTAAATCCGGCGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:473
LEAP-Seq percent confirming:99.7449
LEAP-Seq n confirming:3910
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTCCTAACCTTTACGTCG
Suggested primer 2:TGTAGAGCGTGATGACCTGG