Insertion junction: LMJ.RY0402.038434_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.038434
Chromosome:chromosome_8
Location:4852389
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre08.g384628 antisense 5'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GTAGGGTTGCGACCGCGACGCATTTTGCAG
Internal bar code:GGGGTGTTTTTACGACGCGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:843
LEAP-Seq percent confirming:98.7692
LEAP-Seq n confirming:6741
LEAP-Seq n nonconfirming:84
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:ACCACCTCCAGCTCAATGAC
Suggested primer 2:TGCTGGCTGTGTAGATGGAG