| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.038447 |
| Chromosome: | chromosome_9 |
| Location: | 6541203 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g407801 | AKC1 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTAGTGTCGCTACGGCCTGCTACTCCCTTA |
| Internal bar code: | GCAGCTGCTTCGAAAAGCCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 947 |
| LEAP-Seq percent confirming: | 99.7292 |
| LEAP-Seq n confirming: | 3314 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAATGCGTTTGCTCTGTG |
| Suggested primer 2: | ATTTGTATGTCGGCAGGAGG |