Insertion junction: LMJ.RY0402.038449_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.038449
Chromosome:chromosome_10
Location:2920915
Confidence (%):95
Locus systemic id Locus common name Defline Orientation Feature
Cre10.g440250 PGM19 sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):ATAAAGCGCATTGCGCAAATCGGCATCCAC
Internal bar code:TTGGCCATTATGATTTCGCCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:322
LEAP-Seq percent confirming:99.3939
LEAP-Seq n confirming:164
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GGACTTGCCTCAGTTCTTCG
Suggested primer 2:GACGATGTTCGAGTTGCTGA