| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.038451 |
| Chromosome: | chromosome 1 |
| Location: | 5201767 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036200 | (1 of 1) PF01477//PF07228 - PLAT/LH2 domain (PLAT) // Stage II sporulation protein E (SpoIIE) (SpoIIE) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCGAGCGTCTGATGTCACACTTGTGTT |
| Internal bar code: | GCTTGCCTGTTGGCAGGTAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 922 |
| LEAP-Seq percent confirming: | 93.9124 |
| LEAP-Seq n confirming: | 6001 |
| LEAP-Seq n nonconfirming: | 389 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATATCTGTCAGGGCCACA |
| Suggested primer 2: | AACACAGCAATGAAGGGGAC |