Insertion junction: LMJ.RY0402.038457_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.038457
Chromosome:chromosome_9
Location:1287133
Confidence (%):95
Locus systemic id Locus common name Defline Orientation Feature
Cre09.g399150 sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):CAATGGGGCTCCCCGGAGTCAATACTACAC
Internal bar code:AGTCAGAAAGGGTCCCCGGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:297
LEAP-Seq percent confirming:99.8384
LEAP-Seq n confirming:618
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGGTGACGTTTCTGTGTG
Suggested primer 2:CACACACACACACACACCGT