| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.038464 |
| Chromosome: | chromosome 3 |
| Location: | 1902457 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155051 | SPA1 | Sphinganine-1-phosphate aldolase/lyase; (1 of 1) 4.1.2.27 - Sphinganine-1-phosphate aldolase / Sphingosine-1-phosphate lyase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAACGCGTACACGGTGGCCGAGCCCCAT |
| Internal bar code: | GCCGAACCTAGCAACTAAGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 270 |
| LEAP-Seq percent confirming: | 99.4709 |
| LEAP-Seq n confirming: | 2444 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGGAGTTACCGCATGGAG |
| Suggested primer 2: | CCTCGCAGTTTCCTGACTTC |