| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.038469 |
| Chromosome: | chromosome_8 |
| Location: | 4620299 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g383400 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGACCGTGCGTGGAAGGTGGCGACTGTCG |
| Internal bar code: | GGGCACCGTGGTCCTTGCTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 534 |
| LEAP-Seq percent confirming: | 99.7602 |
| LEAP-Seq n confirming: | 8736 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACAGGATTATTTTGCCGT |
| Suggested primer 2: | CCCTTGCACTCACAGTCGTA |