Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.038472 |
Chromosome: | chromosome_2 |
Location: | 4517265 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g100500 | CYG22 | Adenylate/guanylate cyclase | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATTCCACGGTACGATCGTGGATAAACAAGT |
Internal bar code: | AGAGAGGTCTGCCGGACACTCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 354 |
LEAP-Seq percent confirming: | 98.3064 |
LEAP-Seq n confirming: | 1219 |
LEAP-Seq n nonconfirming: | 21 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGAGGGTGTATTGGGTGCTG |
Suggested primer 2: | COULD_NOT_FIND_PRIMER |