Insertion junction: LMJ.RY0402.038473_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.038473
Chromosome:chromosome_14
Location:1494083
Confidence (%):95
Locus systemic id Locus common name Defline Orientation Feature
Cre14.g618300 sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):TCCTCATCCTACACAGAGGGGGTGGCGGGG
Internal bar code:TCAATTCTAGCTAGAGCGTCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:115
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:58
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGCAAAACTCGTCACTCCAT
Suggested primer 2:GCTTAGAGACGTCTGGACCG