Insertion junction: LMJ.RY0402.038474_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.038474
Chromosome:chromosome 17
Location:3204227
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g722150 PKS3 (1 of 1) 2.3.1.119//2.3.1.199 - Transferred entry: 1.1.1.330, 1.3.1.93, 2.3.1.199 and 4.2.1.134 // Very-long-chain 3-oxoacyl-CoA synthase / Very-long-chain beta-ketoacyl-CoA synthase; Type III polyketide synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGGCGGTACGAGGCGGTACGACGGCGGC
Internal bar code:TTGTACGTGCGGCTATTTCCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:987
LEAP-Seq percent confirming:98.8023
LEAP-Seq n confirming:4702
LEAP-Seq n nonconfirming:57
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:AACCCAAGTAGCCCTCACCT
Suggested primer 2:TGGTCACGTTGATCTTCAGC